I'm using the NEB Mnase kit (Micrococcal Nuclease, 10X Mnase Reaction Buffer, and 100x BSA along with 100 ng of genomic DNA. The final reaction volume is 50 uL and it's incubated in the thermalcycler at 37C for 5 minutes.
I've used a range from 500 to 4000 U in my test but so far I haven't been successful at generating any fragmented product. Am I not using enough starting material? Is there a protocol I can follow to generate the product that I need? Thanks for the help!
I've used a range from 500 to 4000 U in my test but so far I haven't been successful at generating any fragmented product. Am I not using enough starting material? Is there a protocol I can follow to generate the product that I need? Thanks for the help!
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