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  • Using NGS sequences before publishing

    I don't know if this has been encountered before (although it seems very likely) but I am wondering about the conventions or feelings people and editors have about using sequences generated from NGS before publishing a paper on your NGS?

    The lab I am did some RNAseq and assembled our transcript sequences. Great now I have a bunch of transcript sequences to work with (I am into cell bio and RNA bio more then bioinfromatics). We have pushed ahead with additional bioinfromatic analysis on your transcripts but are still a ways away from publishing the results of our NGS results.

    In the mean time I have also used some of the transcript sequences we assembled to do some actual wet lab experiments that are unrelated to our NGS project save for the fact that the sequences I used to construct primers act came from our RNAseq experiments.

    So essentially I would like to know if I have to wait for our NGS paper to be published and then cite that, or if I can reference the fact that we are currently engaged in RNAseq experiments and that this is where my transcript sequences came from.

    In the past my lab has used sequences from an EST library we have been constructing, but we never actually published on its construction. We would simply submit the sequences to NCBI whenever we used them in a publication.

    Have others run into this issue, what did you do? Anyone that serves as an editor or reviewer that has suggestions/comments?

    Thanks
    CC

  • #2
    You could submit sequences from the contigs that you used in your study independently and get accession numbers for them.

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    • #3
      Merely sequencing data with standard protocols isn't often considered publication worthy, so the data tends to get published either with Informatics applications or Biological Analysis. However, to publish a novel sequencing approach before using the data to make some conclusions, might be a good idea.

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