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  • bbduk and kmer masking

    Using bbduk on Illumina PE hg19 fastq files from NextSeq. I have done adapter removal, quality trimming and contamination filtering. Is kmer masking needed or recommended before I align using bbmap? Thank you .

  • #2
    Originally posted by cmccabe View Post
    Using bbduk on Illumina PE hg19 fastq files from NextSeq. I have done adapter removal, quality trimming and contamination filtering. Is kmer masking needed or recommended before I align using bbmap? Thank you .
    Nope! That's just for special use-cases.

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    • #3
      Thank you .

      The bbMap tools are amazingly fast and accurate.

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