Hi, I am new to bioinformatics. Recently, i got my sequence data for WGS using NGS Hiseq 2000. I don't know how to analyze the data. I spent considerable time using Fastqc, velvet 1.2.10 and mauve, but at the end i got a short base of about 140bases, the reference strain has 14mb sequence. I am wondering if the velvet cut the sequences or the raw data was bad.
Please how can i tell the quality of my reads? also, how can i determine the size of my reads?
Please how can i tell the quality of my reads? also, how can i determine the size of my reads?
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