Hi all,
Can we make between library comparisons of peak height? FindPeaks was used to identify sites of transcription factor binding using the same antibody in two different cell types. If peak height is lower in one library than the other at a certain promoter can we infer that different levels of the transcription factor (or histone modification for that matter) are at that locus? would the libaries have to be sequenced to the same depth with the same mapping efficiencies to make any comparison? Does anyone know how reproducible peak heights are between replicate samples?
Any suggestions/discussions/questions welcome!
Thanks
Can we make between library comparisons of peak height? FindPeaks was used to identify sites of transcription factor binding using the same antibody in two different cell types. If peak height is lower in one library than the other at a certain promoter can we infer that different levels of the transcription factor (or histone modification for that matter) are at that locus? would the libaries have to be sequenced to the same depth with the same mapping efficiencies to make any comparison? Does anyone know how reproducible peak heights are between replicate samples?
Any suggestions/discussions/questions welcome!
Thanks
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