What kind of trimming and/or quality filter do you use before mapping reads? Are there any commonly accepted filtering criteria?
I am trying to map some data from an older sequencer and my filtering (Q>15, no more than 6 bases outside quality range allowed) seems to filter out about 85% of the reads.
I am trying to map some data from an older sequencer and my filtering (Q>15, no more than 6 bases outside quality range allowed) seems to filter out about 85% of the reads.
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