SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
CIGAR inconsist with read length. Does Bowtie2 reedit read sequence? wisense Bioinformatics 5 05-26-2015 11:35 PM
Tophat2 read-gap-length and read-mismatches max acceptable values ElizabethRoss RNA Sequencing 0 10-13-2014 03:38 PM
Tophat: options -N --read-edit-dist --read-gap-length Pradhaun Bioinformatics 0 01-04-2013 07:58 AM
a probable mistake in Cuffmerge delasa RNA Sequencing 0 12-07-2012 10:48 AM
picard error: Mismatch between read length and quals length writing read shawpa Bioinformatics 0 08-20-2012 05:52 AM

Reply
 
Thread Tools
Old 03-11-2020, 03:21 AM   #1
ElloisedT
Junior Member
 
Location: Cape Town

Join Date: Mar 2020
Posts: 2
Default Mistake with read length!

Hello!

I erroneously bought an Illumina nano V2 kit 300 cycle kit instead of the 500 cycle kit. I could not return it, and so used it, doing 2 x 150 bp reads.

The problem now is that these reads are not merging because there is no overlap - in fact there is a 160 bp gap. Instead of only using the forward read (150 bp) and losing the reverse read (150 bp), I'm looking for a solution to use both reads and maximise the data I have for accurate OTU assignment.

Any help would be appreciated.

Thank you!
ElloisedT is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:09 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO