Hi guys,
Following sequences are from .sam file (very left several columns). What I do not understand is what is the meaning of second column at the last two lines. Those are
1:N:0:ACTTGA and 1:Y:0:ACTTGA.
I was wondering if anyone can help me with those. Should I ignore these sequences? Thank you very much for you help.
HWI-ST273:295:C0C4PACXX:7:1101:1554:1975 0 chr 1986354 255 50M * 0 NTAATTGTCTCTGCAATGTTATTAACCATAATATCAATTTCACCGAAACG #1=ADDBBFFFHBAGIGEHIFIHIIICGIIGCHGIIIIHIIIIIFDGIIH XA:i:2
HWI-ST273:295:C0C4PACXX:7:1101:1612:1993 0 chr 292259 255 50M * 0 ACTGCTAATGAAGTAACACAAATAGATGGCGTGGCGTCAGTTGATGAAAA @@CDFFFFFHHFHHHGIJJIHHIIICIGIIJFHGHHGGHIJEIJBHGIII XA:i:0
HWI-ST273:295:C0C4PACXX:7:1101:2526:1955 16 chr 135945 255 50M * 0 TCAGTAACGACAGTAAGTTGGCAAGCGACATTAGCCGGTTTAGTAATTGN JJGHFGHIGJJHGGIGDIGIIDJJJJIHHCIJJJJJIHHGHHDFFFD=1# XA:i:1
HWI-ST273:295:C0C4PACXX:7:1101:3762:1995 1:N:0:ACTTGA 4 * 0 0 * * TTGCTTGTTTACCAATGATTAAAAACCATACTTATTTTCAATTTACTGGA @@CFFFFACFHHHGIIIIJJHHJJJJIJIIJJIIJJJJJIIIJJHIIJJD XM:i:0
HWI-ST273:295:C0C4PACXX:7:1101:4204:1964 1:Y:0:ACTTGA 4 * 0 0 * * NTGCTAGTCAATTGCTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT #1DBD+=2CBDDEEEDEIE@FDFEEEEEFEEEIIEDEEA@DDEEDIE? XM:i:0
Following sequences are from .sam file (very left several columns). What I do not understand is what is the meaning of second column at the last two lines. Those are
1:N:0:ACTTGA and 1:Y:0:ACTTGA.
I was wondering if anyone can help me with those. Should I ignore these sequences? Thank you very much for you help.
HWI-ST273:295:C0C4PACXX:7:1101:1554:1975 0 chr 1986354 255 50M * 0 NTAATTGTCTCTGCAATGTTATTAACCATAATATCAATTTCACCGAAACG #1=ADDBBFFFHBAGIGEHIFIHIIICGIIGCHGIIIIHIIIIIFDGIIH XA:i:2
HWI-ST273:295:C0C4PACXX:7:1101:1612:1993 0 chr 292259 255 50M * 0 ACTGCTAATGAAGTAACACAAATAGATGGCGTGGCGTCAGTTGATGAAAA @@CDFFFFFHHFHHHGIJJIHHIIICIGIIJFHGHHGGHIJEIJBHGIII XA:i:0
HWI-ST273:295:C0C4PACXX:7:1101:2526:1955 16 chr 135945 255 50M * 0 TCAGTAACGACAGTAAGTTGGCAAGCGACATTAGCCGGTTTAGTAATTGN JJGHFGHIGJJHGGIGDIGIIDJJJJIHHCIJJJJJIHHGHHDFFFD=1# XA:i:1
HWI-ST273:295:C0C4PACXX:7:1101:3762:1995 1:N:0:ACTTGA 4 * 0 0 * * TTGCTTGTTTACCAATGATTAAAAACCATACTTATTTTCAATTTACTGGA @@CFFFFACFHHHGIIIIJJHHJJJJIJIIJJIIJJJJJIIIJJHIIJJD XM:i:0
HWI-ST273:295:C0C4PACXX:7:1101:4204:1964 1:Y:0:ACTTGA 4 * 0 0 * * NTGCTAGTCAATTGCTACACCATTTAATTGTGGAAGCAAAAGCTAAAGGT #1DBD+=2CBDDEEEDEIE@FDFEEEEEFEEEIIEDEEA@DDEEDIE? XM:i:0
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