I'd like to take an informal poll. How many of you that routinely run a MiSeq have had multiple runs fail because focus is out of spec or Z-axis out of spec. I took over running a MiSeq in Jan, since then I've had 9 runs fail because of focusing issues, most times 200+ cycles into the run.
We typically run 500 cycle kits and have had this issue on both high (genomes) and low (amplicon) complexity samples. Yes Illumina replaces the kit and dispatches a tech but every time we loose a week of machine time. I've talked to a couple of people IRL who have MiSeq and neither have ever had a run fail because of focus. Several times it's seemed that "the" cause of the issue was found-cables rubbing on the stage, etc. But the failures keep happening.
We typically run 500 cycle kits and have had this issue on both high (genomes) and low (amplicon) complexity samples. Yes Illumina replaces the kit and dispatches a tech but every time we loose a week of machine time. I've talked to a couple of people IRL who have MiSeq and neither have ever had a run fail because of focus. Several times it's seemed that "the" cause of the issue was found-cables rubbing on the stage, etc. But the failures keep happening.
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