I had a run on miseq using v3 kit and within an hour the run stopped with the error "no usable signal found in the images it is possible that clustering has failed". I have read that hardware could be an issue but I was just wondering can this happen with wrong library quantification? I did quantified library using Picogreen assay kit and diluted the libraries, however now I feel suspicious about the kit's accuracy.
So I have 2 questions:
1) is it possible that clustering failed that early if the library was not diluted correctly.
2) since clustering didn't even start, what would be the status of that flowcell? I do have an old expired v3 kit I wanted to try but do not have a flowcell for it. So my question is, can this flowcell be reused with another kit since no clustering was started?
Thanks
So I have 2 questions:
1) is it possible that clustering failed that early if the library was not diluted correctly.
2) since clustering didn't even start, what would be the status of that flowcell? I do have an old expired v3 kit I wanted to try but do not have a flowcell for it. So my question is, can this flowcell be reused with another kit since no clustering was started?
Thanks
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