SEQanswers

Go Back   SEQanswers > Applications Forums > Epigenetics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Reduced Representation Bisulfite Sequencing for Methylation Analysis audioPL Illumina/Solexa 10 03-18-2013 06:10 PM
how to order the reads of DNA methylation data from bisulfite sequencing Jim Michael Bioinformatics 7 10-24-2012 05:40 AM
Problems about the Trimmomatic on bisulfite sequencing data sets Jerry_Zhao Bioinformatics 0 04-16-2012 12:02 PM
methylation analysis - bisulfite sequencing with SOLiD? jtjli SOLiD 3 02-10-2009 06:27 AM

Reply
 
Thread Tools
Old 03-13-2013, 02:36 PM   #1
rghan
Junior Member
 
Location: Reno

Join Date: Mar 2011
Posts: 9
Default Is methylation data gathered from bisulfite sequencing noisy between biological reps?

I have a question regarding the noise between biological replicates in bisulfite sequencing experiments. From the papers that I have read, they usually make mention of the number of biological replicates in use (2 in case of the recently published tomato methylome), but they don't, as far as I can tell, mention if there is a great deal of variability in cytosine methylation for a given base. Any information or direction to a paper would be very helpful to me.
rghan is offline   Reply With Quote
Old 03-18-2013, 06:09 AM   #2
dpryan
Devon Ryan
 
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,479
Default

It seems to vary greatly by base and experiment. Check out some of the supplemental figures in this paper, which I recall show some nice methylation level vs. variance graphs.
dpryan is offline   Reply With Quote
Reply

Tags
bisulfite sequencing, epigenetics, methylation calling

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:35 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO