SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing



Similar Threads
Thread Thread Starter Forum Replies Last Post
DESeq analysis with ERCC RNA spike ins swebb Bioinformatics 15 06-14-2018 05:39 AM
ERCC Spike-In Control Suthira RNA Sequencing 9 06-06-2013 01:55 PM
Questions about ERCC RNA Spike-In Control Frazzled Illumina/Solexa 2 06-05-2013 05:17 PM
Normalizing with ERCC spike-in Eric Fournier RNA Sequencing 4 05-14-2013 06:35 AM
ERCC spike in controls for illumina? onconaut Illumina/Solexa 5 11-26-2012 06:47 AM

Reply
 
Thread Tools
Old 02-11-2014, 08:25 AM   #1
jwfoley
Senior Member
 
Location: Stanford

Join Date: Jun 2009
Posts: 181
Default ERCC ExFold vs. regular spike-in mix?

Does anyone know the practical difference between the Ambion ERCC RNA Spike-In Mix (Life Tech #4456740) and their ExFold mixes (#4456739)? The regular mix gets you "absolute quantification", which is nice. I know the ExFold mixes also let you standardize your differential-expression results somehow, but what can you actually do with this information?
jwfoley is offline   Reply With Quote
Old 04-21-2014, 10:22 AM   #2
jparsons
Member
 
Location: SF Bay Area

Join Date: Feb 2012
Posts: 62
Default

The practical difference between the mixes is that most of the spike ins are at different concentrations between mixes.

You can use this difference to determine things - particularly detection limits for varying ratios. This provides you with some valuable ways to prune your gene lists - your spike-ins become "true positives" for DE, in addition to "true negatives".
jparsons is offline   Reply With Quote
Reply

Tags
control, ercc, spike-in

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:04 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO