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Old 01-30-2015, 01:11 PM   #1
jhalpin
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Location: Atlanta, GA

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Question PGM: No loading on 316v2 chip

Hello,

I ran a 316v2 chip today for the 2nd run of an initialization. My chip has 2% loading.. could this be a templating or enrichment failure? My QC percentage was 7.24%. Low, but we've had runs like that succeed.

The first run for the initialization was also a 316v2 chip and had no problems.

Screenshot attached.

Any ideas appreciated

Thanks,

Jessica
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Old 01-30-2015, 01:39 PM   #2
jhalpin
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Angry

So I had a sudden flashback to mixing the melt off solution and thinking it didn't look "sudsy". It's entirely possible I put water in instead of tween, since the bottles are exactly the same. Has anyone else done this? Were your results similar?
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Old 03-06-2015, 07:28 AM   #3
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Default

In case anyone is wondering, it wasn't the tween. I tried to run this pool again yesterday and got the same results. Measured the pool by qubit (should have been ~30ng/mL) and it was too low to read.
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