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Old 08-25-2015, 10:49 AM   #1
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Location: Stanford

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Post phiX on Bioanalyzer

In case anyone else was curious. This is Illumina's ΦX174 reference library ("PhiX Sequencing Control V3", p/n FC-110-3001, allegedly 10 nM), undiluted. As you can see, it's fairly high-MW (460 bp), so smaller libraries might cluster preferentially, and it's actually 8 nM.
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jwfoley is offline   Reply With Quote
Old 08-25-2015, 08:04 PM   #2
Location: San Diego, CA

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Thanks for sharing this data.
I'd just like to add that concentrations of PhiX vary from lot to lot so 8nM wouldn't necessarily be applicable to every tube.
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Old 05-31-2017, 05:26 AM   #3
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Thanks for the info..

Yes the concentration varies a lot.

I have observed it to be between 8-14 nM in different cases.
ritujasaxena is offline   Reply With Quote

bioanalyzer, phix, pooling

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