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Old 01-31-2016, 12:01 PM   #1
Junior Member
Location: San Diego

Join Date: Jan 2016
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Default MiSeq competition?


Just doing a little essay on desktop NGS tech and was wondering if anyone could help me out.

As I understand, the consensus is on the MiSeq system generally for higher throughput and accuracy (homopolymers and indels - still the case?) as compared to the Ion PGM, as well as ease of preparation. With the Ion PGM being the better choice if you have varying amount of samples that need quicker turnaround. With costs essentially the same when it comes down to usable data.

My questions are two-fold: Does this still apply in terms of RNA-seq specifically? And now with the S5, does this change it at all? Or is it just an ease of use improvement.

Also, is there any other desktop NGS system that competes in this space currently available?

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Old 02-10-2016, 06:51 PM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 838

Well, there's the MiniSeq, which is a bit smaller, a bit cheaper (capital cost), and a bit faster, but not really all that useful for people who already have a MiSeq. There's even a competition going for it at the moment:

And, of course, MinION, which currently has an output in the hundreds of megabases for a reasonable flow cell. It's possible Sequel might compete in the low-end high-throughput space as well, depending on how much you are prepared to put down for the sequencer.

Last edited by gringer; 02-10-2016 at 06:53 PM.
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advantage, desktop, ion torrent, miseq

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