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Old 11-12-2010, 09:38 AM   #1
Location: CA

Join Date: Sep 2009
Posts: 11
Default Determining differential expression

I have FPKM values for 3 tissues and no biological replicates (this is 2nd hand data) I want to get a rough idea (giving the lack of replicates) of which genes are differentially expressed prior to doing real time quantitative PCR. Is there anything i should be doing other than looking for fold changes above a specified level?
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