Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Differential Expression Analysis DeDeoxys Bioinformatics 2 09-04-2018 02:43 AM
Normalization, peal calling and differential analysis of ATAC-seq read counts anton.kratz Bioinformatics 0 07-20-2016 09:10 PM
Differential expression results of different read length lincw Illumina/Solexa 6 02-07-2014 07:39 AM
Expression quantification/differential expression gene analysis by RNA-Seq chenjy Bioinformatics 12 08-02-2013 03:06 AM

Thread Tools
Old 12-08-2019, 05:34 PM   #1
Junior Member
Location: Italy

Join Date: Mar 2013
Posts: 6
Default how to treat read-through in mapping for differential expression analysis

hi all
I got 2x150 reads that I used to perform denovo assembly using Trinity, with Trimmomatic option on. Problem is, that nearly 35-40% of reads of all 6 libraries resulted with read-through (Trimmomatic retained forward read only). For assembly that was not a problem, but now I am unsure how to use such libraries to perform differential expression analysis to look for DEGs.
Very thankful to anyone who can give me suggestions
piemmea is offline   Reply With Quote

differential analysis, read-through, rnaseq analysis, trimmomatic

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:33 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO