Hallo to all,
analysing the reads obtained from a Solexa sequencing of a library of small RNAs I obtained a sequence overrepresented (according to fastqc);
this is a sequence of 26 nts matching to the luciferase for the first 23nt, and representing more than 7 milions of reads. The luciferase is not expected to be present in my biological samples so I wonder if there is a step in the sequencing justifying this aspect.
Thank you very much for any help!
Laura
analysing the reads obtained from a Solexa sequencing of a library of small RNAs I obtained a sequence overrepresented (according to fastqc);
this is a sequence of 26 nts matching to the luciferase for the first 23nt, and representing more than 7 milions of reads. The luciferase is not expected to be present in my biological samples so I wonder if there is a step in the sequencing justifying this aspect.
Thank you very much for any help!
Laura
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