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  • #1

    Methyl-Seq Questions

    Hi all,

    I am trying to set up a global methylation project using Agilent's Methyl-Seq system but I'm having some trouble analyzing my sequencing results.

    I was hoping those out there who are also attempting Methyl-Seq could give me their opinion on a few steps that I feel could be the problem:

    How many samples to multiplex? I am currently trying 4.
    How much PhiX DNA to spike in to account for the sequence bias? I have been using 1%.

    Any help would be greatly appreciated,
    Marie
    Tags: agilent, methyl-seq, methylation
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