Hi everyone,
After the QC by qPCR with my 16SrDNA library, I got a concentration value 13.65nM. When I submitted the sample, it was measured as more than 40nM. The sample is planning to be sequenced by illumina. I don't know if the concentration variation is normal? Do you think the sample is good for illumina sequencing? I look forward to your suggestions.Thank you very much!
After the QC by qPCR with my 16SrDNA library, I got a concentration value 13.65nM. When I submitted the sample, it was measured as more than 40nM. The sample is planning to be sequenced by illumina. I don't know if the concentration variation is normal? Do you think the sample is good for illumina sequencing? I look forward to your suggestions.Thank you very much!
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