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Two recent articles suggest that CRISPR/Cas9 can cause mutations in the p53 pathway, irrespective of the target sequence of the guide RNA. The data are extremely interesting since they raise the issue of unpredictable off-target effects that are not directly introduced by the CRISPR/Cas9 complex but rather selected during the genome manipulation process. A thorough analysis of the genomic integrity of cells genetically manipulated with CRISPR is, therefore, the key to successful use of the technology, as described in https://perkinelmer-appliedgenomics.com/research-review-crispr-cas9/ .
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM