Hi everyone,
I am a newbie in genome sequencing and assembly and I need some help.
I had to sequence several bacterial genomes (the biggest is about 5.5 Mb) with Illumina MiSeq as part of my PhD project.
The drafts were assembled by the sequencing company, but I would like to learn the whole process on my own.
Currently, I am learning to use Velvet. I've read the manual and the associated papers but I still cannot figure out how to choose the proper MAXKMERLENGTH value. My reads are 150 bp long (paired-end).
Could you give me an advice?
Thank you for your time in advance.
I am a newbie in genome sequencing and assembly and I need some help.
I had to sequence several bacterial genomes (the biggest is about 5.5 Mb) with Illumina MiSeq as part of my PhD project.
The drafts were assembled by the sequencing company, but I would like to learn the whole process on my own.
Currently, I am learning to use Velvet. I've read the manual and the associated papers but I still cannot figure out how to choose the proper MAXKMERLENGTH value. My reads are 150 bp long (paired-end).
Could you give me an advice?
Thank you for your time in advance.
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