Hi all!
I am trying to correct raw MinION sequencing reads with nanopolish since nanocorrect seems to be deprecated.
However, during the nanopolish workflow found here an alignment to a reference genome seem to be compulsory. So, how to proceed with nanopolish lacking a reference genome? I mean, how to correct raw sequencing reads (or assembled reads) without the bam file obtained with bwa?
Any help will be really apreciated.
I am trying to correct raw MinION sequencing reads with nanopolish since nanocorrect seems to be deprecated.
However, during the nanopolish workflow found here an alignment to a reference genome seem to be compulsory. So, how to proceed with nanopolish lacking a reference genome? I mean, how to correct raw sequencing reads (or assembled reads) without the bam file obtained with bwa?
Any help will be really apreciated.
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