Hello all,
So my lab has done pre-capture pooling using Agilent SureSelect bait libraries before, but that was before they made XT and XT2 kits. We are entertaining the idea of performing pre-capture pooling using and XT library, but with an XT2 reagent kit. I have yet to hear of anyone trying this.
My only caution is I do not know enough about the differences between XT and XT2 (ie. post-capture indexing library and pre-capture indexing library).
If it is just a matter of bait concentration then things should work out ok, assuming we do not multiplex too deeply. I think the max baits to give are 16 anyway.
Anyone have any information/thoughts/experience they could share?
Thanks.
So my lab has done pre-capture pooling using Agilent SureSelect bait libraries before, but that was before they made XT and XT2 kits. We are entertaining the idea of performing pre-capture pooling using and XT library, but with an XT2 reagent kit. I have yet to hear of anyone trying this.
My only caution is I do not know enough about the differences between XT and XT2 (ie. post-capture indexing library and pre-capture indexing library).
If it is just a matter of bait concentration then things should work out ok, assuming we do not multiplex too deeply. I think the max baits to give are 16 anyway.
Anyone have any information/thoughts/experience they could share?
Thanks.
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