Hi,
I understand that the raw HTSeq output is number of reads mapped to each gene (correct me if I'm wrong). Is there an easy way of converting counts to gene expression? The method should take into account gene length as well as total seq depth to be comparable between samples.
Also, I use DESeq2 to do differential expression using HTSeq raw output, is that a valid method? Does DESeq2 automatically take into account gene length?
Thank you!
I understand that the raw HTSeq output is number of reads mapped to each gene (correct me if I'm wrong). Is there an easy way of converting counts to gene expression? The method should take into account gene length as well as total seq depth to be comparable between samples.
Also, I use DESeq2 to do differential expression using HTSeq raw output, is that a valid method? Does DESeq2 automatically take into account gene length?
Thank you!
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