SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
hybridization of custom low concentration ssDNA library pyridine Illumina/Solexa 2 11-14-2013 10:06 PM
custom ssDNA library with strange size distribution of qPCR products pyridine Sample Prep / Library Generation 4 07-17-2013 03:18 AM
custom amplicon sequencing spacesonics Illumina/Solexa 0 08-13-2012 11:54 AM
Generating a Library from 28 nt RNAs... Carlo Sample Prep / Library Generation 1 02-08-2012 10:09 AM

Reply
 
Thread Tools
Old 12-17-2014, 09:55 AM   #1
njh219
Junior Member
 
Location: New York

Join Date: Aug 2013
Posts: 8
Default Generating Custom Sequencing Library

Hi All,
Quick question regarding the orientation of the P5 and P7 regions when generating a sequencing library without using an Illumina kit.
When making forward and reverse primers to generate the library, should they be made so that the P5 and P7 regions are both on one strand, or with the complement of one on the same strand as the other.
example primer set A: 5'-P5-3' 5'-P7-3'
or
example primer set B: 5'-P5-3' 5'-Reverse_Complement_P7-3'
Obviously these primers will include additional elements; I'm not 100% clear on what the orientation of the P5 and P7 regions need to be.
Thanks.
njh219 is offline   Reply With Quote
Old 12-17-2014, 11:52 AM   #2
njh219
Junior Member
 
Location: New York

Join Date: Aug 2013
Posts: 8
Default

Hi all,
After looking into this more I figured out that it acts as in the second example (P5 and the complement of P7 are on the same strand).
Thanks.
njh219 is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:23 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO