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Old 03-03-2011, 04:12 PM   #1
sehrrot
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Default (TruSeq) Quantification before pooling index?

1. Did anyone make library with truseq lib prep kit? How did you quantify 10nM for pooling index? Did you use QuBit or qPCR?

1.1 If you use qPCR, what primer did you use? We've used qPCR primers for paired end lib prep kit (older version) but I am wonder whether we can use same primers in truseq lib prep kit.

Many thanks
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Old 03-03-2011, 04:15 PM   #2
sehrrot
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Also, has anyone done ILMN exome capture? Did you compare the coverage between 6-multiplexing index and just one index?
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Old 03-04-2011, 05:22 AM   #3
upenn_ngs
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For agilent capture we have pooled using equimolar concentrations based on the Bioanalyzer. Read coverage was consistent across indices (within 2%).
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