Hi folks,
For WGBS, we've experienced with human gDNA using Illumina EpiGnome/TruSeq DNA Methylation kit and HiSeq sequencing.
The sequencing duplication rate is dynamic, from <5% to >20%; the more sequencing output we request, the higher duplication rate we get. Is it normal to get 5%-20% of duplication rate?
Can you share your experience on WGBS, which kit you've tried, how much sequencing depth you've requested and how much duplication rate you've got?
Besides, have you seen such "dynamic" duplication rate with different sequencing output?
Thanks,
Wei
For WGBS, we've experienced with human gDNA using Illumina EpiGnome/TruSeq DNA Methylation kit and HiSeq sequencing.
The sequencing duplication rate is dynamic, from <5% to >20%; the more sequencing output we request, the higher duplication rate we get. Is it normal to get 5%-20% of duplication rate?
Can you share your experience on WGBS, which kit you've tried, how much sequencing depth you've requested and how much duplication rate you've got?
Besides, have you seen such "dynamic" duplication rate with different sequencing output?
Thanks,
Wei
Comment