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Old 08-12-2014, 08:45 AM   #1
BerlindaV
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Location: Germany

Join Date: Apr 2014
Posts: 2
Default CDNA synthesis from FFPE: best kit/enzyme?

This is very much a beginner's question, but does anybody have experience with cDNA synthesis on RNA extracted from FFPE? We would like to ligated our own adaptors to the cDNA, so we need a CDNA synthesis system that does both first and second strand synthesis, and that would work on rather poor quality material.

Which producers / kits work best in your experience?

Thank you very much for any advice.
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