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  • Influence of quality of genome assembly for RNA-seq read mapping

    Dear all,
    I am wondering how much the quality of the reference genome assembly influences RNA-seq read mapping (for example Illumina reads mapped to the genome with Tophat). I could not find any published study addressing this question.

    Do you know any paper where RNA-seq mapping rates and quality are compared between model species genomes, draft genomes and de novo assembled genomes?

    Thanks!
    Julien

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