Has anyone done a WFA (SOLiD trial ligation run for assessing the quality of a library) using an old 3.0 WFA reagent kit, but the new 3.5 ("3plus") flowcells/software? We just did one and got terrible results on a library that previously worked well. (New ePCR, same library).
We are going to try a new WFA and include a quad with left over beads that we know are good. But since we noticed that the WFA reagent tubes were drained dry by the autosampler, whereas we expected some liquid to be left over because the new, lower-profile, flow cells should require less reagents, I thought I would ask here.
--
Phillip
We are going to try a new WFA and include a quad with left over beads that we know are good. But since we noticed that the WFA reagent tubes were drained dry by the autosampler, whereas we expected some liquid to be left over because the new, lower-profile, flow cells should require less reagents, I thought I would ask here.
--
Phillip
Comment