Hi everyone,
I have attempted to perform indexing-first ChIP (iChIP) as described here: http://www.ncbi.nlm.nih.gov/pubmed/25103404. The way this protocol works is you perform a total H3 IP, then you index the DNA using the illumina adaptor and unique barcodes, then you pool the indexed chromatin and perform an H3K27Ac IP on the pooled chromatin.
After submitting for sequencing, my barcodes were found in the sample, but additional Illumina barcodes were also found. I never even purchased the barcodes that were found so I was unsure how they might have ended up in my sample?
Does this seem like a contamination at my sequencing facility, or is there any other way for other barcodes to end up in your sample?
I have attempted to perform indexing-first ChIP (iChIP) as described here: http://www.ncbi.nlm.nih.gov/pubmed/25103404. The way this protocol works is you perform a total H3 IP, then you index the DNA using the illumina adaptor and unique barcodes, then you pool the indexed chromatin and perform an H3K27Ac IP on the pooled chromatin.
After submitting for sequencing, my barcodes were found in the sample, but additional Illumina barcodes were also found. I never even purchased the barcodes that were found so I was unsure how they might have ended up in my sample?
Does this seem like a contamination at my sequencing facility, or is there any other way for other barcodes to end up in your sample?
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