First post here. Just getting into NGS after many years doing all things molly bolly.
I'm reading the Illumina 'Preparing Samples for Paired-End Sequencing' protocol and get to the 'Purify Ligation Products' section which suggests using gel-purification, EthBr and UV exposure - ugh! I certainly wouldn't want my precious DNA anywhere near ethidium bromide and UV!
In the past I've used Clontech Chromaspin size-selection columns to selectively remove salts, dNTPs, oligo's, etc from various reaction mixes, etc. There are different cut-off sizes and they've always worked very well for me. It would seem that the Chromaspin 100 columns would work well here in place of gel-purification.
Anyone tried them?
I'm reading the Illumina 'Preparing Samples for Paired-End Sequencing' protocol and get to the 'Purify Ligation Products' section which suggests using gel-purification, EthBr and UV exposure - ugh! I certainly wouldn't want my precious DNA anywhere near ethidium bromide and UV!
In the past I've used Clontech Chromaspin size-selection columns to selectively remove salts, dNTPs, oligo's, etc from various reaction mixes, etc. There are different cut-off sizes and they've always worked very well for me. It would seem that the Chromaspin 100 columns would work well here in place of gel-purification.
Anyone tried them?