HiSeq 4000 : typical RAW data output in GBytes

sklages · 09-14-2016, 04:36 AM

Hi,

can someone provide some info on how much raw data (in terms of GBytes, not Gbases) is generated for some typical HiSeq 4000 WGS runs (150,75, whatever)? How much fastq data (GBytes) is generated roughly/typically?

We need to plan computing and storage resources accordingly.

best,
Sven

kcchan · 09-14-2016, 04:21 PM

From the Illumina FAQ:

Quote:

The run folder size for the maximum read length of 2 x 150 is about 0.6 TB.

sklages · 09-15-2016, 12:16 AM

Quote:

Originally Posted by kcchan

From the Illumina FAQ:

Sure. For our HiSeq 2000 a few years ago, we had folder sizes up to 4TB; Illumina stated in their FAQ that a run folder is roughly 1TB.

I am just curious how real-world numbers align with Illumina's FAQ.

GenoMax · 09-15-2016, 09:23 AM

Some recent run data folder sizes for HiSeq 4000.

SE 50 bp - 90G
PE 50 bp - 170G
PE 75 bp - 250G
PE 150 bp - 500G

sklages · 09-15-2016, 11:29 PM

GenoMax, thanks for the numbers. That makes me optimistic :-)

GenoMax · 09-16-2016, 02:06 AM

Should have said that those are raw data folder sizes before bcl2fastq v.2.x conversion.

sklages · 09-16-2016, 02:21 AM

Quote:

Originally Posted by GenoMax

Should have said that those are raw data folder sizes before bcl2fastq v.2.x conversion.

That's what I was interested in :-)

But btw, what's the fastq output for such runs (roughly)?

GenoMax · 09-17-2016, 03:16 AM

Quote:

Originally Posted by sklages

That's what I was interested in :-)

But btw, what's the fastq output for such runs (roughly)?

Fastq folder size (sample dependent)

PE 50 bp - ~195 G
PE 75 bp - ~275 G
PE 150 bp - ~350-400 G

Brian Bushnell · 09-17-2016, 10:54 AM

Are those numbers for gzipped or raw fastqs?

GenoMax · 09-17-2016, 08:05 PM

For gzipped fastq's.

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09-14-2016, 04:36 AM	#1
sklages Senior Member Location: Berlin, DE Join Date: May 2008 Posts: 628	HiSeq 4000 : typical RAW data output in GBytes Hi, can someone provide some info on how much raw data (in terms of GBytes, not Gbases) is generated for some typical HiSeq 4000 WGS runs (150,75, whatever)? How much fastq data (GBytes) is generated roughly/typically? We need to plan computing and storage resources accordingly. best, Sven

09-15-2016, 09:23 AM	#4
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,080	Some recent run data folder sizes for HiSeq 4000. SE 50 bp - 90G PE 50 bp - 170G PE 75 bp - 250G PE 150 bp - 500G

09-15-2016, 11:29 PM	#5
sklages Senior Member Location: Berlin, DE Join Date: May 2008 Posts: 628	GenoMax, thanks for the numbers. That makes me optimistic :-)

09-16-2016, 02:06 AM	#6
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,080	Should have said that those are raw data folder sizes before bcl2fastq v.2.x conversion.

09-17-2016, 10:54 AM	#9
Brian Bushnell Super Moderator Location: Walnut Creek, CA Join Date: Jan 2014 Posts: 2,707	Are those numbers for gzipped or raw fastqs?

09-17-2016, 08:05 PM	#10
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,080	For gzipped fastq's.