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Old 03-09-2018, 05:08 AM   #1
Location: Netherlands

Join Date: Sep 2014
Posts: 24
Talking bacterial RNAseq using TruSeq Lib prep

Hi all. I am in the process of starting RNAseq from bacterial samples and I am trying to identify the best strategy to do it. Given that bacterial RNA is not poly-adenylated, a random hexamer-based system is essential. Has anyone used the TruSeq stranded library prep kit for bacterial RNA? Or do you have a good kit to recommend? The samples will be run on the MiSeq or NextSeq systems. Any advice to guide me through the magical world of RNAseq will be much appreciated
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Old 03-13-2018, 02:15 AM   #2
Location: Montpellier (France)

Join Date: May 2008
Posts: 93

We used the Truseq kit succesfully on Bacteria. 2 things are essential:
- DNAse treatment of your RNA.
- Good depletion efficiency for the ribosomal RNA. Ribozero kit are usually doing well.
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