Rapid Library Adapter Dimer

Br3ndan · 03-29-2011, 10:44 PM

Hi All,

We have recently done a 454 GS junior run of a rapid library build of some low-copy number aDNA and had an overwhelming number of reads appearing to be dimerisation of our y-adapters. Has anyone else experienced this?

Thanks

lalremruata · 05-05-2011, 05:36 AM

What i did was made aDNA library using RAPID lib Kit. Amplified with emPCR primer and then cloned the amplified library to ensure fragment-adaptor ligation. of course my purpose was to enrich using NimbleGen array prior to 454 GS junior sequencing!!

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03-29-2011, 10:44 PM	#1
Br3ndan Junior Member Location: Perth WA Join Date: Mar 2011 Posts: 1	Rapid Library Adapter Dimer Hi All, We have recently done a 454 GS junior run of a rapid library build of some low-copy number aDNA and had an overwhelming number of reads appearing to be dimerisation of our y-adapters. Has anyone else experienced this? Thanks

05-05-2011, 05:36 AM	#2
lalremruata Junior Member Location: Germany Join Date: Oct 2010 Posts: 1	What i did was made aDNA library using RAPID lib Kit. Amplified with emPCR primer and then cloned the amplified library to ensure fragment-adaptor ligation. of course my purpose was to enrich using NimbleGen array prior to 454 GS junior sequencing!!