Newbie...Help needed!!

Beta1 · 01-23-2012, 03:22 AM

I am a newbie to sequencing but mainly working on the DNA prep side of things.
Can anyone recommend a good thread to inform me on steps needed for DNA library preparation (intended for illuminia platform) and the important factors to consider for sequencing?
Also, a glossary for terms such as read length, paired end reads, A-overhang, enrichment etc etc
I keeping coming across these terms but now idea what they mean and why they are important.
Any good recommendation for basic reading material on DNA library prep and sequencing would be much appreciated.

jjohnson · 01-23-2012, 10:21 AM

I would start by signing up for webinars through Illumina. They cover many of the topics below. You just need to create an account and enter your serial number of your machine to get started. If you don't have access to a machine, and Illumina FAS or sales rep should be able to point you towards this info as a potential customer.

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01-23-2012, 03:22 AM	#1
Beta1 Junior Member Location: Ireland Join Date: Jan 2012 Posts: 2	Newbie...Help needed!! I am a newbie to sequencing but mainly working on the DNA prep side of things. Can anyone recommend a good thread to inform me on steps needed for DNA library preparation (intended for illuminia platform) and the important factors to consider for sequencing? Also, a glossary for terms such as read length, paired end reads, A-overhang, enrichment etc etc I keeping coming across these terms but now idea what they mean and why they are important. Any good recommendation for basic reading material on DNA library prep and sequencing would be much appreciated.

01-23-2012, 10:21 AM	#2
jjohnson Member Location: Washington DC Metro Area Join Date: Aug 2009 Posts: 20	I would start by signing up for webinars through Illumina. They cover many of the topics below. You just need to create an account and enter your serial number of your machine to get started. If you don't have access to a machine, and Illumina FAS or sales rep should be able to point you towards this info as a potential customer. __________________ Justin H. Johnson \| Twitter: @BioInfo \| LinkedIn: http://bit.ly/LIJHJ \| EdgeBio