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Old 08-21-2009, 03:15 PM   #1
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Default Solicitations Regarding the Recent Upgrades to 120 tile format


I am trying to get a sampling of peoples experiences concerning the recent "upgrades" on the GAII machines that incorporate the new reagent/cooling system. We have several machines which used to function marvelously. Since Illumina started sending people out to "upgrade" them they have never quite worked right. We see much worse map'ability on 2 x 76 human RNA-Seq libraries suggesting base calling issues, and the signals are dropping dramatically during the course of the runs. (They are not overloaded, 12 million clusters and v 1.4 software). In short, we got much better results when we had to use fresh reagents that we changed more frequently.

We are inclined to think it is either mechanical or chemical in nature. I'm wondering if it has to do with the new cooling/reagent system that has recently been installed?

What have other people's experiences with the upgrades been? Love 'em hate 'em?
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Old 08-24-2009, 03:55 PM   #2
Location: Baltimore, MD

Join Date: Jun 2009
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You post caught our interest as we are about to upgrade our GAII machine. It's rather new as its only been functional for a few weeks. Can you share more details with us? For example, when you say:
the signals are dropping dramatically during the course of the runs
how many bases can you effectively use? 2x50?

Thank you!
L. Collado Torres, Ph.D. student in Biostatistics.
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