SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Illumina's newest upgrades (a response to Life Tech?) GW_OK Illumina/Solexa 38 01-20-2012 04:17 AM
FASTQC for checking quality of 120 bp reads madsaan Bioinformatics 4 06-07-2011 12:17 AM
GenomeQuest Upgrades Online Patent Searching GenomeQuest Vendor Forum 0 05-24-2010 08:35 AM
non-homogeneous background on tile images Erwin Illumina/Solexa 0 03-13-2010 08:21 AM
Sample tile images across a lane ScottC Illumina/Solexa 4 07-06-2008 03:14 PM

Reply
 
Thread Tools
Old 08-21-2009, 03:15 PM   #1
data_overload
Junior Member
 
Location: USA

Join Date: Aug 2009
Posts: 1
Default Solicitations Regarding the Recent Upgrades to 120 tile format

Hello.

I am trying to get a sampling of peoples experiences concerning the recent "upgrades" on the GAII machines that incorporate the new reagent/cooling system. We have several machines which used to function marvelously. Since Illumina started sending people out to "upgrade" them they have never quite worked right. We see much worse map'ability on 2 x 76 human RNA-Seq libraries suggesting base calling issues, and the signals are dropping dramatically during the course of the runs. (They are not overloaded, 12 million clusters and v 1.4 software). In short, we got much better results when we had to use fresh reagents that we changed more frequently.

We are inclined to think it is either mechanical or chemical in nature. I'm wondering if it has to do with the new cooling/reagent system that has recently been installed?

What have other people's experiences with the upgrades been? Love 'em hate 'em?
data_overload is offline   Reply With Quote
Old 08-24-2009, 03:55 PM   #2
lcollado
Member
 
Location: Baltimore, MD

Join Date: Jun 2009
Posts: 65
Default

Hello,

You post caught our interest as we are about to upgrade our GAII machine. It's rather new as its only been functional for a few weeks. Can you share more details with us? For example, when you say:
Quote:
the signals are dropping dramatically during the course of the runs
how many bases can you effectively use? 2x50?

Thank you!
__________________
L. Collado Torres, Ph.D. student in Biostatistics.
lcollado is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:33 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO