|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Library QC- cntrl loci enrichment doesn't match pre and post library generation | yogioner | Sample Prep / Library Generation | 0 | 12-03-2012 04:40 PM |
| Cuffmerge assemblies on pre and post intervention ? | muehlsch | Bioinformatics | 2 | 08-31-2012 07:06 AM |
| Blocking oligos in MiSeq kit | LSAD | Sample Prep / Library Generation | 0 | 04-13-2012 02:09 AM |
| Pre- and Post-mRNA fragmentation electropherograms | grassgirl | 454 Pyrosequencing | 15 | 01-13-2012 02:43 PM |
| Sure Select pre-Hyb lib storage | EGraf | Sample Prep / Library Generation | 0 | 10-26-2011 07:06 AM |
 |
|
|
Thread Tools |
10-31-2013, 09:05 AM
|
#1 |
|
Member
Location: UK Join Date: Nov 2010
Posts: 49
|
lack of blocking primer in pre and post hyb...
Hi,
It looks like I made a mistake in the hybridization library prep. Following the Rohland2012 protocol I forgot to use truncaded primers for pre-hyb (6 cycles). I used the fully indexed and flow cell ready primers for this step (no blocking primers added either)... After the post-hyb amplification I was able to see a product, but after the sequencing was completed almost exclusively illumina adapters were present. Does it look like a showcase for daisy-chaining PCR product ? What are my options with regards to library ? Can I simply go to post hyb library left over and use blocking primers ? Shall I go back before pre-hyb ? Thanks for any help! |
|
|
|
11-06-2013, 03:09 AM
|
#2 |
|
Senior Member
Location: Amsterdam Join Date: Nov 2009
Posts: 112
|
I would do the hyb again with the blockers.
|
|
|
|
|
| Thread Tools | |
|
|
