Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Tot Newsbot! Literature Watch 0 11-09-2011 03:10 AM
Illumina directional RNA-seq protocol Herve Illumina/Solexa 10 06-13-2010 08:18 AM
Homemade Illumina mRNA-SEQ protocols kbushley Sample Prep / Library Generation 2 04-20-2010 06:37 PM
illumina alternative v1.5 protocol for small rna seq vs. the standard protocol ik76 Sample Prep / Library Generation 1 03-25-2010 03:24 PM
mRNA-Seq protocol Xi Wang Illumina/Solexa 5 11-05-2009 07:41 AM

Thread Tools
Old 01-02-2010, 07:13 AM   #1
Xi Wang
Senior Member
Location: MDC, Berlin, Germany

Join Date: Oct 2009
Posts: 317
Default Illumina mRNA-seq protocol

Hi all,

I found in the Illumina mRNA-Seq protocal that usually only ~200bp frangments are sequenced. Since random priming could synthesize cDNA fragments from anywhere of the mRNA fragments, if the synthesized cDNA fragments are much shorter than 200bp, the cDNA fragments will be filtered out by gel selection, and thus those positioins of transcripts can never be sequenced. Is my understanding right? And if so, this sample preparing would introduce bias, right?

Thanks in advance.
Xi Wang
Xi Wang is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:56 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO