NEBNext GAII Paired End Prep

TonyBrooks · 03-23-2010, 10:11 AM

Does anyone have any experience of preparing a 300bp Paired End library for sequence capture using the NEB NEBNext reagents and custom PE adapter oligos?

GW_OK · 03-25-2010, 03:40 PM

I prepped a paired end library for SureSelect using NEB reagents and Illumina adapter oligos. Worked fine. Gonna try the custom adapters next.

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03-23-2010, 10:11 AM	#1
TonyBrooks Senior Member Location: London Join Date: Jun 2009 Posts: 298	NEBNext GAII Paired End Prep Does anyone have any experience of preparing a 300bp Paired End library for sequence capture using the NEB NEBNext reagents and custom PE adapter oligos?

03-25-2010, 03:40 PM	#2
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 411	I prepped a paired end library for SureSelect using NEB reagents and Illumina adapter oligos. Worked fine. Gonna try the custom adapters next.