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Old 08-15-2014, 03:02 PM   #1
Location: California

Join Date: Sep 2008
Posts: 45
Default TruSeq PCR-Free odd peaks

We've used the TruSeq PCR-Free kit on very short fragments, 170bp, and are getting a double peak when looking at the results on the bioanalyzer (attached). Has anyone seen this before?
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Old 08-15-2014, 09:30 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

If the trace is for adapter ligated fragments, this could be due to structure of adapters. You can do a PCR with small aliquot of library and check the size of amplicons.

Last edited by nucacidhunter; 08-16-2014 at 12:07 AM.
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bioanalyzer, truseq, truseq pcr free

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