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08-15-2014, 03:02 PM
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#1 |
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Member
Location: California Join Date: Sep 2008
Posts: 45
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TruSeq PCR-Free odd peaks
We've used the TruSeq PCR-Free kit on very short fragments, 170bp, and are getting a double peak when looking at the results on the bioanalyzer (attached). Has anyone seen this before?
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08-15-2014, 09:30 PM
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#2 |
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Jafar Jabbari
Location: Melbourne Join Date: Jan 2013
Posts: 1,238
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If the trace is for adapter ligated fragments, this could be due to structure of adapters. You can do a PCR with small aliquot of library and check the size of amplicons.
Last edited by nucacidhunter; 08-16-2014 at 12:07 AM. |
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| bioanalyzer, truseq, truseq pcr free |
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