Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA -> cDNA libraries on PacBio snf Pacific Biosciences 3 01-23-2015 10:26 AM
Truseq cDNA libraries show sharp peak of cDNA BUFtoBOS Sample Prep / Library Generation 2 09-23-2014 09:00 AM
Werid RNA traces- bump above 28S - can I make libraries from this? Noa Sample Prep / Library Generation 1 11-08-2013 01:37 PM
What should be included to make a good transcriptome/RNA-seq paper? poisson200 Bioinformatics 5 06-27-2011 07:39 AM
PubMed: Construction of small RNA cDNA libraries for deep sequencing. Newsbot! Literature Watch 0 01-07-2011 11:30 AM

Thread Tools
Old 12-19-2014, 05:39 AM   #1
Junior Member
Location: Japan

Join Date: Dec 2014
Posts: 1
Default Good way to make cDNA libraries for one-embryo RNA-seq

I am a undergraduate student and planning to conduct single-embryo RNA-seq.
The developmental stage of samples are various, from blastula (about 1k cells) to just before hatching.
(I am using zebrafish, X.laevis and chick.)
Could I make cDNA libraries in the same way?

Some members in my lab are performing Quartz-seq, so there are necessary reagents.
What I would like to know is…

1. Whether I could perform Quartz-seq with late stage embryo.
(too many impurities, or RNAs?)

2. or, could I make cDNA libraries without amplification?
(especially eary stage embryo)

If I should verify myself, I would be glad to know how to do.

thank you very much.
Yui is offline   Reply With Quote

cdna library, embryo, sequencing

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:36 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO