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Old 12-19-2014, 05:39 AM   #1
Yui
Junior Member
 
Location: Japan

Join Date: Dec 2014
Posts: 1
Default Good way to make cDNA libraries for one-embryo RNA-seq

Hi!
I am a undergraduate student and planning to conduct single-embryo RNA-seq.
The developmental stage of samples are various, from blastula (about 1k cells) to just before hatching.
(I am using zebrafish, X.laevis and chick.)
Could I make cDNA libraries in the same way?

Some members in my lab are performing Quartz-seq, so there are necessary reagents.
What I would like to know is…

1. Whether I could perform Quartz-seq with late stage embryo.
(too many impurities, or RNAs?)

2. or, could I make cDNA libraries without amplification?
(especially eary stage embryo)

If I should verify myself, I would be glad to know how to do.



thank you very much.
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cdna library, embryo, sequencing

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