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Old 01-17-2017, 08:46 PM   #1
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Location: Chengdu

Join Date: Jan 2017
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Question How indexes are added in TruSeq Custom Amplicon v1.5?

Hi folks. Could someone tell me that how indexes and sequencing primers are added in TruSeq Custom Amplicon v1.5 library preparation? By ligation (to the end of custom probe 1/2) or by base pairing (hybridize to custom probe 1/2)? Any answer appreciated!
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Old 01-18-2017, 07:40 AM   #2
Location: Montpellier (France)

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Posts: 93

Judging by the picture you joined to your message, they are added during the PCR step.
Indexing during PCR is rather common ine Illumina's protocols especially when for amplicons and when using Nextera.

Last edited by huguesparri; 01-18-2017 at 07:41 AM. Reason: typo
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illumina, library, targeted amplicons, targeted enrichment, targeted resequencing

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