cluster density

andersej · 12-12-2012, 11:38 AM

Is it possible that a high cluster density >1300K/mm^2 that may result in difficulties for the MiSeq software to distinguish/misidentify neighboring clusters to intorduce errors i.e. the sequence diverge (maybe due to SNV or indel) and the software cannot identify the correct sequence and this results in a frame shift call?

swbarnes2 · 12-12-2012, 02:28 PM

If 20 reads, from 20 different clusters, going in both directions and all starting from different positions in the genome, and all say there's a frameshift, you have a frameshift. It would be a mistake to conclude anything from a single read, if that's what you are asking.

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12-12-2012, 11:38 AM	#1
andersej Junior Member Location: USA Join Date: Dec 2012 Posts: 1	cluster density Is it possible that a high cluster density >1300K/mm^2 that may result in difficulties for the MiSeq software to distinguish/misidentify neighboring clusters to intorduce errors i.e. the sequence diverge (maybe due to SNV or indel) and the software cannot identify the correct sequence and this results in a frame shift call?

12-12-2012, 02:28 PM	#2
swbarnes2 Senior Member Location: San Diego Join Date: May 2008 Posts: 912	If 20 reads, from 20 different clusters, going in both directions and all starting from different positions in the genome, and all say there's a frameshift, you have a frameshift. It would be a mistake to conclude anything from a single read, if that's what you are asking.