Custom TruSeq-equivalent WGS library preps, for high number of smal genomes

WilliamIsMyUserName · 04-24-2020, 03:38 AM

Does anyone have experience with custom high troughput "TruSeq-equivalent WGS library preps"?

Normal TruSeq library prep costs around somewhere between $300 to $150 per sample. Some providers offer custom high troughput"TruSeq-equivalent WGS library preps" for 48 or 96 sample plates at a cost of around $60 per sample.

Especially for small genomes (smaller than 3Gb, or even smaller than 1Gb), library prep is a big part of the total cost. So it would be interesting to know if these custom protocols give equivalent results.

Does anyone have experience with these custom "TruSeq-equivalent WGS library preps"? Or know a recent review paper?

luc · 04-24-2020, 09:26 PM

Who is advertising this?
Hard to tell what it means - there are soo many different library prep chemistry options.

WilliamIsMyUserName · 04-25-2020, 01:00 AM

Ah ok.

It is listed on the Cornell price list website under "High-throughput Library preparation for Illumina Sequencing":
http://www.biotech.cornell.edu/brc/g...ces/price-list

Is there a good recent overview/review of what currently the library prep options are for Illumina whole genome sequencing?
That show what the trade offs are regarding quality, time and cost?

Just for whole genome sequencing, excluding targeted / reduced complexity sequencing.

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04-24-2020, 03:38 AM	#1
WilliamIsMyUserName Junior Member Location: Europe Join Date: Apr 2020 Posts: 3	Custom TruSeq-equivalent WGS library preps, for high number of smal genomes Does anyone have experience with custom high troughput "TruSeq-equivalent WGS library preps"? Normal TruSeq library prep costs around somewhere between $300 to $150 per sample. Some providers offer custom high troughput"TruSeq-equivalent WGS library preps" for 48 or 96 sample plates at a cost of around $60 per sample. Especially for small genomes (smaller than 3Gb, or even smaller than 1Gb), library prep is a big part of the total cost. So it would be interesting to know if these custom protocols give equivalent results. Does anyone have experience with these custom "TruSeq-equivalent WGS library preps"? Or know a recent review paper?

04-25-2020, 01:00 AM	#3
WilliamIsMyUserName Junior Member Location: Europe Join Date: Apr 2020 Posts: 3	Ah ok. It is listed on the Cornell price list website under "High-throughput Library preparation for Illumina Sequencing": http://www.biotech.cornell.edu/brc/g...ces/price-list Is there a good recent overview/review of what currently the library prep options are for Illumina whole genome sequencing? That show what the trade offs are regarding quality, time and cost? Just for whole genome sequencing, excluding targeted / reduced complexity sequencing. Last edited by WilliamIsMyUserName; 04-25-2020 at 05:56 AM.

04-24-2020, 09:26 PM	#2
luc Senior Member Location: US Join Date: Dec 2010 Posts: 452	Who is advertising this? Hard to tell what it means - there are soo many different library prep chemistry options.