Does ASCII 64 to 104 eq ASCII offset 33?

[louis7781x]

Hi,

I have illumina pipeline 1.3 RNA-seq paired-end data,I want to use bwa on galaxy.

The bwa on galaxy said it Must have Sanger-scaled quality values with ASCII offset 33 .

I know Illumina Pipeline 1.3 and 1.4. Using a Phred scale using ASCII 64 to 104,I don't understand can I run it ?

I am confused with Phred scale using ASCII 64 to 104 and ASCII offset 33

[essvee]

In galaxy, I believe you can convert to ASCII offset 33 with the fastq groomer.