Hi!
I'm brand new at this, and I have been trying to use the makeblastdb command on TACC (tried both lonestar and stampede). I have the blast modules loaded. I have downloaded the protein sequences from the genomes of several animals from the NCBI ftp site and eventually would like to blast my de novo transcriptome against the protein databases for annotation purposes.
I have used the makeblastdb command before with no problems, but for the past two days I've been stumped because it is not giving me the correct output files even when i rerun a makeblastdb command that was previously successful.
my command line looks like this:
$ makeblastdb -in protein.fa -dbtype prot -out Fugu.prot.db
instead of getting a Fugu.prot.db output file, i'm getting three binary files:
Fugu.prot.db.phr
Fugu.prot.db.pin
Fugu.prot.db.psq
any ideas on what to try next?
Thanks!!!!
I'm brand new at this, and I have been trying to use the makeblastdb command on TACC (tried both lonestar and stampede). I have the blast modules loaded. I have downloaded the protein sequences from the genomes of several animals from the NCBI ftp site and eventually would like to blast my de novo transcriptome against the protein databases for annotation purposes.
I have used the makeblastdb command before with no problems, but for the past two days I've been stumped because it is not giving me the correct output files even when i rerun a makeblastdb command that was previously successful.
my command line looks like this:
$ makeblastdb -in protein.fa -dbtype prot -out Fugu.prot.db
instead of getting a Fugu.prot.db output file, i'm getting three binary files:
Fugu.prot.db.phr
Fugu.prot.db.pin
Fugu.prot.db.psq
any ideas on what to try next?
Thanks!!!!
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