Hi all,
My most recent data set consists of a set of fastq files that have already been demultiplexed by Casava 1.8. However, my sequencing core told me that this only allows for a single bp mismatch. Because my barcodes are rather long many reads end up in the unknown indices. However, I designed the barcodes to allow for 5 errors between them and I would now like to utilize this. I can easily look at the fastq files and see the index in the read name header, but I can not find a program that will allow me to demultiplex based on this format. My core doesn't keep the original reads, so I am stuck having to demultiplex reads that have already been demultiplexed by Casava. If anyone knows of a program that can accomplish this that would be great.
My most recent data set consists of a set of fastq files that have already been demultiplexed by Casava 1.8. However, my sequencing core told me that this only allows for a single bp mismatch. Because my barcodes are rather long many reads end up in the unknown indices. However, I designed the barcodes to allow for 5 errors between them and I would now like to utilize this. I can easily look at the fastq files and see the index in the read name header, but I can not find a program that will allow me to demultiplex based on this format. My core doesn't keep the original reads, so I am stuck having to demultiplex reads that have already been demultiplexed by Casava. If anyone knows of a program that can accomplish this that would be great.
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