Tweet
I am doing some experiment using BowTie and WHAM which are short read aligners that aligns short DNA sequences (reads) to the human genome. In WHAM, it is specified that for 75 bps reads, it is biologically required to allow matching with two errors and if the read length increases, allowed mismatch errors should increase. Now, I want to experiment with those two tools on 150 bps. Can anybody help me what should be the allowed matching errors for 150 bps or where can I find information about it ? Actually, I want information about, "maximum tolerated miss match errors" which is biologically relevant or for various (specially 150 bps) read length. I became confused because of this list of aligners, which has varied "maximum tolerated miss match errors". Can anybody please help me about this ? Thanks in advance.
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM